CCNA1-specific T cells are polyfunctional and cytotoxic. (A) Representative IFN-γ ICS data from 1 donor (left), and summary data for all responding donors (right; mean ± standard error of the mean; n = 11). (B) ICS analysis of dual (IFN-γ and TNF-α) cytokine secreting cells (representative data, left; summary data [n = 11], right). (C) ICS analysis of donor 8’s cell line gated on CD8⁺ T cells, showing CCNA1 specificity in the CD8 compartment. (D) These specific cells lysed autologous CCNA1 peptide-pulsed PHA blasts in a MHC class I–restricted manner, as assessed in a 4-hour Cr⁵¹ cytotoxicity assay at a range of effector/target (E:T) ratios. (E) Cytotoxic activity from 2 additional donor cell lines against autologous pulsed and unpulsed PHA blasts at an E:T 20:1 ratio.