Figure 1.
![CCNA1-specific T-cell expansion and characterization. (A) Fold expansion of CCNA1-specific T cells (mean ± standard error of the mean [SEM]), based on cell counting using trypan blue exclusion (n = 12). (B) Immunophenotype of the expanded cell lines as assessed by flow cytometric analysis. (C) Specificity of expanded T cells as determined by IFN-γ ELISpot assay and data are presented as SFC/1 × 105 input cells. The whiskers of the plot indicate minimum and maximum values; boxes indicate median and interquartile ranges.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/4/2/10.1182_bloodadvances.2019000715/3/advancesadv2019000715f1.png?Expires=1724943440&Signature=1Be5WuOXkSvbtZDviNdXrglLDukwgEf-lHPHhTrrUMKbgsblc9vIwnyEIaKpHAaDyeK76biAS2goxSqcUGbxtqoMrM9KeS~6sFhCGG5wcaUoLgmIvhRaoqo3Hp-BC7jWUrnGKiMiNv~Lm73Hvhrh7Aow4VmwO0t100my~OYTSfVVqj5dkproND4N-gQXcfj~D5k5hULkyboKgd2vrrQpBTq4Lj7QWwSzYO3--tGsGhMJOubPwLkUsTZlYUw4rbxJ0GxaNiSpCtWVMVsugyvc35wZTFZSz2eMxC7tCRE08GePaMZ7s2LTzVSVv1K-yTiKuSXIL0Aoeh4PRegvTNVlug__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CCNA1-specific T-cell expansion and characterization. (A) Fold expansion of CCNA1-specific T cells (mean ± standard error of the mean [SEM]), based on cell counting using trypan blue exclusion (n = 12). (B) Immunophenotype of the expanded cell lines as assessed by flow cytometric analysis. (C) Specificity of expanded T cells as determined by IFN-γ ELISpot assay and data are presented as SFC/1 × 105 input cells. The whiskers of the plot indicate minimum and maximum values; boxes indicate median and interquartile ranges.