Phf6 deletion enriched differentiation and cell cycle–associated functions in HSPCs. (A) Enrichment Map showing the significantly perturbed functions in Phf6-null compared with wild-type HSPCs nodes are Gene Ontology gene sets, and edges indicate shared genes. (B) GSEA plot for representative gene sets enriched in Phf6 KO vs wild-type cells. (C) Heatmap representation of oncogenic signatures significantly enriched or depleted (P < .05) in Phf6 KO HSPCs. (D) GSEA plot for representative oncogenic signatures enriched in Phf6 KO cells vs wild-type cells. E2F1, Myc oncogene (MYC), and MTOR signatures were positively enriched in Phf6 KO LT-HSCs, MPP2, and MPP3. ER, endoplasmic reticulum.