The synergistic effects of Phf6 deletion on ICN1 overexpression. (A) Six weeks after transplantation, the recipients of Phf6 KO+ICN1 cells had lower hemoglobin (HGB) levels. (B) The recipients of Phf6 KO+ICN1 cells had higher percentages of CD4⁺CD8^– cells within the ICN1 overexpressing BMCs (P = .0325). Representative FACS plots of bone marrow are shown. (C) The recipients of Phf6 KO+ICN1 cells had larger spleens. (D) The recipients of Phf6 KO+ICN1 cells had a shorter overall survival (P < .0001). (E) The survival disadvantages of Phf6 KO+ICN1 mice held true with different cell doses. (F) There were more LICs (CD4^–CD8^–CD25⁺CD127⁺) in Phf6 KO+ICN1 mice marrow. (G) Phf6 KO+ICN1 mice had more LIC (P < .0001) according to limiting dilution assays. *P < .05.