Transplantation assays of Phf6 knockout (KO) BMCs. (A) Chimerism was higher in the marrow of recipients transplanted with bulk Phf6 KO BMCs (P = .0312; wild-type, n = 7; Phf6 KO, n = 7). The recipients transplanted with sorted LSK cells from bone marrow of Phf6 KO mice also had significantly higher chimerism compared with wild-type (P = .0478; wild-type, n = 9; Phf6 KO, n = 8). (B) In a competitive repopulation unit assay, the recipients of Phf6 KO BMCs had higher percentages of Lin^–, LK, GMP, and megakaryocyte-erythroid progenitor (MEP) cells (P = .0065, .000169, .0037, and .0032, respectively; wild-type, n = 7; Phf6 KO, n = 7) among the donor-derived cells. (C) Recipients of Phf6 KO BMCs had higher WBC and neutrophil (NEUT) counts but lower lymphocyte (LYMPH) counts than those of wild-type BMCs. (D) Within donor-derived blood cells, recipients of Phf6 KO BMCs had a lower proportion of CD4⁺ and CD8⁺ cells but higher CD11b⁺Gr1^– cells in the blood compared with wild-type BMCs. (E) The chimerism in blood (left panel) and marrow (right panel) of the recipients of Phf6 KO BMCs was further enhanced in the secondary transplantation. Representative FACS plots of bone marrow are shown. *P < .05; **P < .01; ***P < .001; ****P < .0001. CMP, fluorescence-activated cell sorting analysis and cell sorting; PB, peripheral blood.