Cell counts in the peripheral blood of mice 8 to 12 weeks of age. (A) Western hybridization of BMCs harvested from 3 wild-type mice (left 3 lanes) and 3 Phf6 knockout (KO) mice (right 3 lanes). Phf6 (∼41 kDa) was markedly depleted in the bone marrow of Phf6 KO mice. (B) Female Phf6 KO mice had higher WBC, lymphocyte (LYMPH), and monocyte (MONO) counts than wild-type littermates. (C) Phf6 KO mice (n = 19), male and female, had higher B220⁺ B-cell counts (left panel), lower CD4⁺ T-cell counts (middle panel), and lower CD8⁺ T-cell counts (right panel) in their peripheral blood than wild-type mice (n = 17). *P < .05; **P < .01; ***P < .001; ****P < .0001. BASO, basophil; CBC, complete cell count; EOS, eosinophil; NEUT, neutrophil.