Murine models of granulopoiesis. (A) BM (n ≥ 4) was stimulated in vitro at 37°C for 30 minutes with PBS (P), GM-CSF (GM), G-CSF (G), PMA, MCP-1 (M), RANTES (R), IL-6, LPS (L), or pHrodo (pH), fixed, and analyzed by flow cytometry. One-way ANOVA was performed with the post hoc Fisher's LSD test. ****P < .0001. (B-C) PBS, GM-CSF, or G-CSF were introduced by tail-vein injection (n = 4). pPMN percentages in BM and blood were determined after 1 hour. Representative contour plots are shown. One-way ANOVA was performed with the post hoc Fisher's LSD test. *P < .05; **P < .01.