pPMNs in BM, blood, and peritoneum fluctuate with acute inflammation. BM (A), blood (B), and peritoneal (C) PMNs were monitored after peritoneal injection of E coli–pHrodo bioparticles (100 µL). (D) pPMNs were determined as a percentage of total PMNs in BM, blood, and peritoneum at each time point, based on high expression of CD66a and CD11b. Mean percentages of pPMNs ± SEM are shown (n ≥ 9). P values were determined by ANOVA with a post hoc Fisher's LSD test. All comparisons are with untreated controls (T = 0 m). *P < .0001. (E) Blood and peritoneal lavage were collected 1 hour after tail-vein injection of SYTOX Green and pHrodo-induced peritonitis, and analyzed by flow cytometry. Representative SYTOX histograms of blood PMNs (Bl), peritoneal PMNs (P), and FMO controls are indicated. The mean percentages of SYTOX-positive blood and peritoneal PMNs ± SEM are indicated; n = 4. A 2-tailed Student t test was performed. **P < .005.