SPOP depletion specifically and strongly induces γ-globin in HUDEP-2 cells. (A) RNA-seq analysis of cell pools with SPOP sgRNA#1, -#2, and -#3. Each dot represents an individual gene. Each gene is depicted according to FPKM (fragments per kilobase per million) value. HBG1/2 indicates the combined levels of HBG1 and HBG2. (B) Scatter plots showing the results of mass spectrometry analysis of cell pools with SPOP sgRNA#1, -#2, and -#3 using whole-cell lysates. Data represent log₂ protein abundances. Protein abundances were quantified by tandem mass tag–labeled mass spectrometry. Each dot represents a protein.