Acute depletion of DCs does not increase CD8 T-cell motility. Irradiated 129 mice were reconstituted with BM from B6 F1(CD11c-YFPxCD11c-DTR) mice, wt B6 CD4 cells and B6 RFP⁺ CD8 cells. Approximately 28 days after bone marrow transplantation, mice were treated with PBS or with DT 48 and 24 hours before imaging. (A) Still images from representative videos before DT (left panel; supplemental Video 13) or after DT (right panel; supplemental Video 14). CD8⁺ T-cell mean speeds (B), displacements (C), and sphericity (D) before and after DT treatment. *P < .0001.