Mature DCs have far greater expression levels of MHC-II and costimulatory molecules. Surface expression of HLA-DR, CD40, and CD80 on freshly isolated neutrophils (T = 0), neutrophils plus RBC-ops cultured for 20 hours (T = 20), monocytes, immature or mature DCs. Data represent the MFI values of HLA-DR (A), CD40 (B), and CD80 (C) (mean ± SEM; n = 3-5). Asterisks above the straight-line bars represent significant differences compared with T = 0 neutrophils; asterisks above the inverted U–shaped spanner bars represent significant differences between the indicated bars (****P < .0001; **P < .01; *P < .05).