Changes in histone modifications upon loss of JMJD1C. (A) Representative snapshots of RNA-seq and H3K36me3 ChIP-seq (triplicate and duplicate biological replicates, respectively; 1 representative replicate is shown) results in Jmjd1c sgRNA vs Renilla sgRNA cells: Irf8 and Gas7 are genes repressed by JMJD1C, and Actb is a control gene. (B) Heat map of differentially expressed genes (adjusted P < .05 and fold change > 1.5) between MLL-AF9 cells harboring Renilla vs JMJD1C sgRNAs. (C) Venn diagram of overlapping differentially regulated genes in the knockout and sgRNA RNA-seq data set. P < 8.5⁻⁹⁷, exact hypergeometric test. GSEA enrichment plots of differentially regulated genes between Jmjd1c^f/f and Jmjd1c^−/− cells²⁹  (D), indicated gene sets (E, top and bottom) in MLL-AF9 cells harboring Renilla (Renilla) vs JMJD1C sgRNAs (REST) RNA-seq data. (F) GSEA enrichment plots of differentially regulated genes between MLL-AF9 cells harboring Renilla vs JMJD1C sgRNAs in H3K36me3 ChIP-seq data of Renilla vs JMJD1C sgRNAs (REST).