Analysis of GloFli fish thrombocytes by flow cytometry. Representative dot plots show the presence of GFP⁺ and RFP⁺ thrombocytes in whole blood of Glo fish (A), Fli fish (B), GloFli fish (C), and GloCD41 fish (D). FL1 and FL2 channels are shown as FL1-A and FL2-A on the x-axis and y-axis, respectively. Forward scattering in FL1 and FL2 channels measures green and red fluorescence from GFP⁺ and RFP⁺ thrombocytes and is shown in Q4-LR and Q4-UL gates, respectively. DP thrombocytes (GFP⁺/RFP⁺) are shown in the Q4-UR gate. The percentages of thrombocytes are shown in the respective gates. The large percentage of cells shown in Q4-LL (lower left quadrangle) gates represents other blood cells. Gating was according to the fluorescence intensities of thrombocytes in the side scattering.