Figure 4.
EBV infection elicits type I IFN production in huNSG mice. huNSG mice were infected with 5 × 105 RGU EBV, and blood samples were analyzed 18 hours postinfection. IFN-α (A) or IL-12p40 (B) production was measured in the serum by enzyme-linked immunosorbent assay (PBS, n = 11; EBV, n = 12). (C) IFN-α levels were compared in the serum of huNSG mice that were PBS treated (n = 6) or infected with either 5 × 105 RGU of wild-type EBV (wt, n = 6), EBNA3A-deficient EBV (3aKO, n = 6), or EBNA3C-deficient EBV (3cKO, n = 8). (D) ISG induction was measured in blood leukocyte lysates by using quantitative real-time polymerase chain reaction. n = 8 for PBS; n = 9 for EBV. Unpaired Student t tests were applied.