DWJM increases CXCR4 cell surface expression and enhances CD34⁺cell transmigration. (Ai) Contour plots of CD34 and CXCR4 expression on CD34⁺ cells. (Aii) Percentage of CD34⁺CXCR4⁺ cells from 3 independent experiments (n = 3, data are expressed as mean ± SEM, **P < .01, ***P < .001, ****P < .0001). (Bi) Gating strategy: debris exclusion → dead cell exclusion → MFI analysis of CXCR4 expression on gated CD34⁺ cells. (Bii) CXCR4 expression on gated CD34⁺CD34⁺ cells at baseline (red, day 0), culture without DWJM (green, day 7), and culture with DWJM (purple, day 7). (Biii) Summary of CXCR4 expression on CD34⁺ cells. (Biv) Transwell assay (n = 3, data are expressed as mean ± SEM, *P < .05, **P < .01). DAPI, 4′,6-diamidino-2-phenylindole; FSC, forward scatter; SCC, side scatter.