DWJM maintains the CD34⁺CD38⁻phenotype and increases c-Kit expression of CD34⁺cells. (Ai) Expression of CD38 on CD34⁺ cells after 7-day culture under 6 culture conditions. (ii) Frequency of CD38⁺ cells. (iii) CD38 MFI on gated CD38⁺ cells. (B) c-Kit expression in CD34⁺ cells under 6 conditions. HPSCs were stained with 1 antibody composed of CD34, CD38, and c-Kit. (i) Gating strategy. Percentages of c-Kit⁺ cells were analyzed in CD34⁺CD38⁺ (top) and CD34⁺CD38⁻ (bottom) cell subsets. (ii) Summary of c-Kit⁺ percentages in CD34⁺, (iii) CD34⁺CD38⁻, and (iv) CD34⁺CD38⁺ cell subsets under 6 conditions for 7 days (data from 3 independent studies, expressed as mean ± SEM, *P < .05, **P < .01). N.S., not significant.