DWJM decreases the proliferation rate and maintains primitive phenotypes of CD34⁺cells. (Ai) The percentage of cells surviving using Trypan blue exclusion assay in 4 cell culture conditions after 7-day culture. (Aii) Quantification of cell proliferation rate by CellTrace Violet intensity assay. UCB CD34⁺ cells cultured under 4 conditions were collected and labeled with CellTrace Violet. Cell proliferation rate was accessed by flow cytometry after labeling. (B) Double staining of CellTrace Violet and CD34 in 4 culture conditions. (C) Quantification of apoptosis and necrosis of hematopoietic cells on 4 culture conditions by AnnexinV/PI staining. Results, shown as density plots (left), are quantified in bar graphs (right). Data shown are expressed as mean ± SEM, n = 3 from 3 independent studies, *P < .1. Ctrl, control.