CMML-specific gene signature in NUP98-HBO1–transduced human CD34⁺cells. (A) Schematic diagram of the experimental strategy for gene expression array analysis. (B) GSEA plot showing the ranked NUP98-HBO1–induced genes in CB CD34⁺ cells. NUP98-HBO1–induced genes were tested in the data set of PB CD34⁺ cells from patients with CMML (n = 15) and HDs (n = 4) (E-MTAB-1044). (C) GSEA plot showing the ranked NUP98-HBO1–induced genes in CB CD34⁺ cells. NUP98-HBO1–induced genes were tested in the data set of BM CD34⁺ cells from patients with MDS (n = 26) and HD (n = 4) (GSE43399) or MDS (n = 183) and HD (n = 17) (GSE19429). (D) Scatter plot of NUP98-HBO1–induced genes in CB CD34⁺ cells. (E-F) Ranked monocytic gene signature (E) and granulocytic gene signature (F) were tested in the data set of NUP98-HBO1–induced genes in CB CD34⁺ cells.