HSP47⁺myofibroblasts accumulated in fibrotic lesions of the lacrimal gland after allogeneic BMT. BALB/c mice were transplanted with 8 × 10⁶ BM cells plus 2 × 10⁷ splenocytes from allogeneic (Allo) B10.D2 or syngeneic (Syn) BALB/c donors on day 0, following 6 Gy TBI. Lacrimal glands were harvested on day +35 after BMT. H&E-stained (A) and MT-stained (B) images of the lacrimal glands. Scale bars, 100 μm. (C) MT staining (upper panels) and immunofluorescent images (lower panels) for HSP47 (red), with DAPI nuclear staining (blue) on the pairs of consecutive serial sections. Areas in the rectangles (middle panels) were magnified (right panels). Scale bars, 50 μm. Crosses indicate glandular parenchyma. Arrows indicate HSP47⁺ fibroblasts. (D) Immunofluorescent staining of HSP47 (green) and α-SMA (red) with DAPI nuclear staining (blue). Arrows indicate HSP47⁺ myofibroblasts. Scale bars, 50 μm. (E) Proportion of fibrotic area stained with MT compared with the total area of the sections of lacrimal glands from Syn (n = 6) and Allo (n = 9) mice. (F) The amount of collagen deposition in the lacrimal glands from Syn (n = 7) and Allo (n = 7) mice was normalized with tissue weight. Data from 2 independent experiments were combined and are shown as mean ± SEM. (G) The volume of tear secretion was measured as the length of wet cotton threads from Syn (n = 8) and Allo (n = 9) mice on day +35 posttransplant. Data from 2 independent experiments were combined and are shown as mean ± SEM. Dashed lines indicate the borders between the glandular parenchyma and interstitium. *P < .05, **P < .01, ***P < .005. Du, ductal lumen.