pF8 differentially drives GFP expression in liver and spleen. Representative immunofluorescence analysis of mouse liver (A) and spleen (B) of C57BL/6 HA, 1 and 6 months after LV.pF8.GFP injection. Scale bars, 50 μm. (C) Flow cytometry analysis showing GFP expression pattern in liver NPCs and hepatocytes isolated from C57BL/6 mice 1 month after LV.pF8.GFP delivery. (D) Evaluation of LSEC markers Tie-2, CD146, and CD-31 on GFP⁺ cells of the NPC fraction.