Identification of putative and alternative TSSs in human F8 gene. (A) Zenbu Genome Browser view of human F8 gene locus. Genomic coordinates in hg19 chromosome X are shown on top. Genes and transcripts are color-coded according to their orientation in the genome (positive strand, green; negative strand, purple). A track with annotated reference sequence for each gene at this locus is included (RefSeq). Expression across the whole collection of FANTOM5 human libraries is shown (F5 human Cap Analysis of Gene Expression, N = 1829). Green and purple arrowheads indicated transcription initiation in sense and antisense orientation, respectively. (B) TSS1-driven expression of F8 in human tissues (fetal, adult) measured by F5 Cap Analysis of Gene Expression analysis. (C) In silico analysis of pF8 sequence predicts the presence of several TFBS belonging to TFs expressed in hepatocytes and hematopoietic and endothelial cells. In parentheses are the numbers of nucleotides (nt) recognized.