Figure 3.
Figure 3. IL-34 accelerates MM-induced osteoclast formation in vitro. (A) Relative expression of Csf1r, Il34, or Rankl mRNA in the CD45+ myeloid compartment, CD45− stromal compartment, or MOPC315.BM cells (normalized to Gapdh). (B) Protein levels of IL-3, IL-6, IL-34, and TNFα in the supernatants of the indicated cultures. (C) qRT-PCR analysis of Trap, Dc-stamp, Oc-stamp, or Cathepsin K in BM cells differentiated in the presence of the indicated cultures. (D) Representative photomicrographs (TRAP stain; original magnification ×5) of BM cells differentiated for 10 days in the presence of supernatants of BMSCs-MOPC315.BMControl or BMSCs-MOPC315.BMIl34KD cocultures. Yellow arrows indicate TRAP+ multinucleated cells. Data are mean ± SEM; *P < .05. N.D., not determined; N.S., not significant.

IL-34 accelerates MM-induced osteoclast formation in vitro. (A) Relative expression of Csf1r, Il34, or Rankl mRNA in the CD45+ myeloid compartment, CD45− stromal compartment, or MOPC315.BM cells (normalized to Gapdh). (B) Protein levels of IL-3, IL-6, IL-34, and TNFα in the supernatants of the indicated cultures. (C) qRT-PCR analysis of Trap, Dc-stamp, Oc-stamp, or Cathepsin K in BM cells differentiated in the presence of the indicated cultures. (D) Representative photomicrographs (TRAP stain; original magnification ×5) of BM cells differentiated for 10 days in the presence of supernatants of BMSCs-MOPC315.BMControl or BMSCs-MOPC315.BMIl34KD cocultures. Yellow arrows indicate TRAP+ multinucleated cells. Data are mean ± SEM; *P < .05. N.D., not determined; N.S., not significant.

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