Cytokine production. (A) Cytokine production in the T cell subsets. Production of IL-17 was high in the microenvironment in tumors and TILs. IL-4 production was elevated only in the tumor population. Tumor and TILs were capable of producing IFN-γ. IL-10 production occurred in a handful of tumor cases, but the median IL-10 production was highest in the CD4 TIL population. (B) tSNE clustering of cytokine data, which shows the T helper cell subsets of the immune response. This tumor was a producer of IL-17 and IL-4. *P < .05; **P < .01; ***P < .001; ****P < .0001.