NF-κB activation and cell-cycle dysregulation in ATL and control T cells. Whole cell lysates were prepared as reported⁶  and analyzed by standard immunoblotting using the indicated antibodies. (A) Evaluation of NF-κB pathway activation. (B) Evaluation of cyclin-dependent kinase inhibitor, cyclin, and CDK expression. Each immunoblot shown used the same protein lysates; the β-actin control in panel B is applicable to panel A. Each blot was repeated ≥5 times with the same and different lysates.