Safety in HA animals overexpressing rat FVIIa. (A-E) Complete blood counts in HA, WT, HA-AAV <708 (transgene expression in nanograms per milliliter) and HA-AAV ≥708 (transgene expression in nanograms per milliliter) are shown: red blood cells (RBC), white blood cells (WBC), platelets (PLT), hemoglobin (Hb), and hematocrit (HCT) were monitored weekly throughout the study. (F) Fibrinogen (FIB) levels were monitored weekly in the same animal cohorts as in (A-E). The following symbols are used for each group in all panels: ▼, WT controls (n = 12); ▲, HA controls (n = 11); ●, HA – AAV ≥708 (transgene expression in nanograms per milliliter; n = 15); ▪, HA-AAV <708 (transgene expression in nanograms per milliliter; n = 18). Arrow indicates time of AAV administration. No statistical differences were observed among the experimental groups (repeated measures 1-way ANOVA with Tukey’s multiple comparison test). All data are presented as mean ± SD.