Tail vein transection to test for efficacy of expressed rat FVIIa. (A) Total volume of blood loss (milliliters) during a 45-minute period after tail vein injury. Each symbol represents an individual animal in each designated animal cohort. (B) The data in panel A are plotted as a function of transgene expression (ng/mL) vs total volume of blood loss (mL). Each symbol represents an individual animal. Gray horizontal bar indicates the range of blood loss values of WT control animals. Dashed lines indicate the 708 and 1250 ng/mL levels. ****P < .0001; **P < .01, using a 1-way ANOVA with Tukey’s multiple comparison test. All data are presented as mean ± SD.