Quantitative bone histomorphometry of skeletally mature WT and F8^TKOmale mice. Histomorphometric indices of bone formation and resorption were quantified in femurs of 6-month-old male WT and F8^TKO mice. Dynamic osteoblast measurements were made in calcein-labeled unstained plastic-embedded sections and static indices in sections stained with VonKossa/MacNeals Tetrachrome. The following osteoclast indices were quantified in deplasticized sections stained for tartrate-resistant acid phosphatase and counterstained with fast green: (A) tissue area, (B) bone area, (C) BV/TV, (D) MAR, (E) BFR/BS, (F) representative images of double calcein-labeled bone sections. Magnification: 20× objective (original magnification ×200 final), (G) osteoblast number (N.Ob), (H) osteoblast surface (Ob.S), (I) osteoclast number (Oc.N)/BS, and (J) osteoclast surface (Oc.S)/BS. Data are expressed as boxplots (median + IQR) with Tukey whiskers. n = 10 WT and 10 F8^TKO mice/group. *P < .05, **P < .01, and ****P < .0001 by Student t test.