RUNX1 binding in model cells and primary AMLs. (A) Heat map of RUNX1mut-binding sites in RUNX1mut-transduced CB cells, control and RUNX1mut-induced iPSCs, and primary AMLs with RUNX1mut. (B) Screenshot of RUNX1 binding in CB-RUNX1mut and control cells, iPSC-RUNX1mut cells with and without dox, and primary AMLs expressing RUNX1mut. (C) Differential expression of genes that are bound by RUNX1 in both RUNX1wt and RUNX1mut AMLs. NUCB2 (left) is higher expressed in RUNX1wt AMLs, whereas TAL1 (right) is expressed more in RUNX1mut AMLs. Differential levels of H3K27ac are associated with the levels of expression. (D) Expression levels and levels of DNaseI, H3K27ac, and H3K27me3 at the CTSG locus in RUNX1wt vs RUNX1mut AMLs. (E) Expression levels and levels of DNaseI, H3K27ac, and H3K27me3 at the CEBPA locus in RUNX1wt vs RUNX1mut AMLs.