American Society of Hematology

Figure 5.

$Figure 5. The RUNX1mut gene program. (A) PCA plot using RNA-seq results of the indicated cell types. Hollow circle indicates RNA-seq data from published primary AMLs carrying RUNX1mut (https://leucegene.ca/research-development/). Normal cells are indicated within the blue circles. (B) Based on RNA-seq predicted average fraction of CD34+ cells, granulocytes, monocytes, and macrophages in RUNX1mut-expressing cells, using CIBERSORT. (C) Differential expression of monocytic and granulocytic signature genes in normal CD34 cells, RUNX1mut expressing CB cells, iPSC models, and primary AML cells. (D) Heat map of gene expression by k-means clustering in RUNX1mut-expressing CB, iPSC, and primary AML cells vs control CD34+ cells resulting in 4 main clusters. (E) Biological process enrichment for each of the 4 clusters identified in panel D. **P < .01; ***P < .001. ns, not significant.$

The RUNX1mut gene program. (A) PCA plot using RNA-seq results of the indicated cell types. Hollow circle indicates RNA-seq data from published primary AMLs carrying RUNX1mut (https://leucegene.ca/research-development/). Normal cells are indicated within the blue circles. (B) Based on RNA-seq predicted average fraction of CD34⁺ cells, granulocytes, monocytes, and macrophages in RUNX1mut-expressing cells, using CIBERSORT. (C) Differential expression of monocytic and granulocytic signature genes in normal CD34 cells, RUNX1mut expressing CB cells, iPSC models, and primary AML cells. (D) Heat map of gene expression by k-means clustering in RUNX1mut-expressing CB, iPSC, and primary AML cells vs control CD34⁺ cells resulting in 4 main clusters. (E) Biological process enrichment for each of the 4 clusters identified in panel D. **P < .01; ***P < .001. ns, not significant.

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