iPSC differentiation is impaired when expressing RUNX1mut. (A) Time schedule of iPSC differentiation protocol and marker expression analysis. At the beginning of the hematological differentiation (blue arrow), doxycycline is added, inducing expression of RUNX1mut. Cell surface marker expression on CD34/CD45 was performed on different points (red arrows). (B) Example of marker analysis and a representable figure of CD34⁺/CD45⁺ and CD34⁻/CD45⁺ expression on different points during differentiation. (C) Marker analysis and a representable figure of CD15 and CD16 expression in control vs RUNX1mut-expressing iPSCs on day 17 of differentiation. (D) Log2 expression levels of CEBPA in control vs dox-induced iPS cells.