CEBPA expression is decreased in RUNX1mut cells. (A) CD114 expression of CB control vs RUNX1mut cells in time in cells cultured with G-CSF. (B) qRT-PCR analysis of CEBPA, an SPI1 expression in control vs RUNX1mut after ∼2 weeks of expansion in liquid culture medium. (C) Relative CEBPA expression in RUNX1wt vs RUNX1mut AMLs in the TCGA data set. AML1-ETO or inv(16) AMLs are excluded from this analysis. (D) Immature marker (CD34) and immature markers (CD14 and CD15) expression before and after 3 days of overexpression of CEBPA-ER induced by tamoxifen. (E) Percentage of mature and immature-appearing cells in RUNX1mut and RUNX1mut + CEBPA cells after 3 days of overexpression of CEBPA. Two hundred cells were score a rated mature or immature on the basis of their morphology. *P < .05; **P < .01; ***P < .001.