American Society of Hematology

Figure 2.

$Figure 2. RUNX1mut-transduced CB CD34+ cells have increased growth potential, LTC-IC-frequency, and CD34+ maintenance. (A) Relative expansion in MS5 coculture of control vs RUNX1mut. Growth curve of MS5 coculture (5 weeks). After 5 weeks, the MS5 was replated (see supplemental Figure 2A for continued growth). (B) Marker expression analysis of adherent fraction of RUNX1mut cells in MS5 coculture after 10 weeks. Expansion of suspension fraction was very limited and did not allow for surface marker expression after 10 weeks. (C) Relative expansion in liquid culture over a course of several weeks in control vs RUNX1mut (n = 3). (D) Marker expression analysis of RUNX1mut cells in liquid culture after 10 weeks. (E) CD34+ expression of 3 CB control vs RUNX1mut cells in time. Each line represents a different culture. (F) LTC-IC frequency of control vs RUNX1mut cells. *P < .05.$

RUNX1mut-transduced CB CD34⁺cells have increased growth potential, LTC-IC-frequency, and CD34⁺maintenance. (A) Relative expansion in MS5 coculture of control vs RUNX1mut. Growth curve of MS5 coculture (5 weeks). After 5 weeks, the MS5 was replated (see supplemental Figure 2A for continued growth). (B) Marker expression analysis of adherent fraction of RUNX1mut cells in MS5 coculture after 10 weeks. Expansion of suspension fraction was very limited and did not allow for surface marker expression after 10 weeks. (C) Relative expansion in liquid culture over a course of several weeks in control vs RUNX1mut (n = 3). (D) Marker expression analysis of RUNX1mut cells in liquid culture after 10 weeks. (E) CD34⁺ expression of 3 CB control vs RUNX1mut cells in time. Each line represents a different culture. (F) LTC-IC frequency of control vs RUNX1mut cells. *P < .05.

This Feature Is Available To Subscribers Only