American Society of Hematology

Figure 1.

$Figure 1. RUNX1mut increases replating capacity. (A) Schematic overview of RUNX1wt and RUNX1 S291fs300X mutant. (B) CFC and replating potential of RUNX1mut vs control. At the start, 500 cells were plated per dish in duplicates. Between days 10 to 14, the number of colonies were scored and all colonies were harvested. For each replating, 25 000 cells per dish were plated. Replating potential of control vs RUNX1mut in the CD14−/CD15− fraction (C) and replating potential of control vs RUNX1mut in the CD14+/CD15+ fraction (D). At the start, 500 cells were plated per dish in duplicates. Between days 10 and 14, the number of colonies were scored and colonies were harvested and stained for CD14 and CD15. In a new CFC, 25 000 CD14+ or CD15+ cells and 25 000 CD14−/CD15− cells were FACS sorted. After 10 to 14 days, this was repeated for another replating round. *P < .05; **P < .01.$

RUNX1mut increases replating capacity. (A) Schematic overview of RUNX1wt and RUNX1 S291fs300X mutant. (B) CFC and replating potential of RUNX1mut vs control. At the start, 500 cells were plated per dish in duplicates. Between days 10 to 14, the number of colonies were scored and all colonies were harvested. For each replating, 25 000 cells per dish were plated. Replating potential of control vs RUNX1mut in the CD14⁻/CD15⁻ fraction (C) and replating potential of control vs RUNX1mut in the CD14⁺/CD15⁺ fraction (D). At the start, 500 cells were plated per dish in duplicates. Between days 10 and 14, the number of colonies were scored and colonies were harvested and stained for CD14 and CD15. In a new CFC, 25 000 CD14⁺ or CD15⁺ cells and 25 000 CD14⁻/CD15⁻ cells were FACS sorted. After 10 to 14 days, this was repeated for another replating round. *P < .05; **P < .01.

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