New LSC detection in MA9 mice. HSC markers were used to detect leukemic HSC/HPC cells in the BM of MA9 mice. GFP⁺ cells were first gated. Lin⁻IL7-R⁻CD34^+/−c-Kit⁺Sca-1⁺CD150⁻CD41⁻Mac-1⁺ (L-CD34^−/+LSK) cells were detected in all MA9 leukemic mice derived from 5 different populations (HSC1 [A], HSC2 [B], HPC1 [C], HPC2 [D], and HPC3 [E]). SSC, side scatter.