American Society of Hematology

Figure 1.

$Figure 1. Reconstitution potentials of HSC1, HSC2, HPC1, HPC2, and HPC3. (A) Flow cytometry sorting strategy for HSC and HPC populations. Lin− cells were separated into CD34− and CD34+ fractions and then gated on c-Kit+Sca-1+ cells, respectively. According to the expression levels of CD150 and CD41, CD34−LSK cells were separated into HSC1, HSC2, and HPC1, and CD34+ LSK cells were separated into HPC2 and HPC3. (B) The percentage of donor cells in PB. Ten HSC1, 10 HSC2, 10 HPC1, 50 HPC2, and 250 HPC3 cells were mixed with 5 × 105 BM cells and injected into lethally irradiated recipient mice. The PB of recipient mice was analyzed at 1, 4, 6, and 12 months after transplantation (n = 6 for each population; however, some mice died after transplantation).$

Reconstitution potentials of HSC1, HSC2, HPC1, HPC2, and HPC3. (A) Flow cytometry sorting strategy for HSC and HPC populations. Lin⁻ cells were separated into CD34⁻ and CD34⁺ fractions and then gated on c-Kit⁺Sca-1⁺ cells, respectively. According to the expression levels of CD150 and CD41, CD34⁻LSK cells were separated into HSC1, HSC2, and HPC1, and CD34⁺ LSK cells were separated into HPC2 and HPC3. (B) The percentage of donor cells in PB. Ten HSC1, 10 HSC2, 10 HPC1, 50 HPC2, and 250 HPC3 cells were mixed with 5 × 10⁵ BM cells and injected into lethally irradiated recipient mice. The PB of recipient mice was analyzed at 1, 4, 6, and 12 months after transplantation (n = 6 for each population; however, some mice died after transplantation).

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