Figure 4.
Neutralization of VEGFR2 blocks the effect of FG-4497 on G-CSF–induced HSPC mobilization. (A) Mice were administered G-CSF for 2 days, FG-4497 or vehicle for 3 days, and neutralizing rat anti-mouse VEGFR2 mAb DC101 or control rat IgG1 every other day. At harvest, the numbers of CFCs (B-C), LKS+ HSPCs (D-E), and LKS+CD48−CD150+ HSCs (F-G) were measured in peripheral blood (B,D,F) and spleen (C,E,G). Data are from 2 pooled experiments performed several months apart. Each point is an individual mouse. Bars are means ± standard deviation. *P < .05, **P < 10−2, ***P < 10−3. G+DC, G-CSF with vehicle DC101 mAb; G+F+DC, G-CSF plus FG-4497 plus DC101; G+F+IgG, G-CSF plus FG-4497 plus rat IgG1; G+IgG, G-CSF with vehicle with rat IgG1.