Figure 1.
VEGFR tyrosine kinase inhibitor PTK787 inhibits synergistic effect of FG-4497 on G-CSF–induced HSPC mobilization. (A) Mice were administered G-CSF for 2 days, FG-4497 or vehicle for 3 days, and PTK787 or vehicle for 3 days. At harvest, the numbers of CFCs (B-C), LKS+ HSPCs (D-E), and LKS+CD48−CD150+ HSCs (F-G) were measured in peripheral blood (B,D,F) and spleen (C,E,G). Data are from 2 pooled experiments performed several months apart. Each point represents an individual mouse. Bars are means ± standard deviation. *P < .05, **P < 10−2, ***P < 10−3, ****P < 10−4. G, G-CSF with vehicles; G+F, G-CSF plus FG-4497; G+F+P, G-CSF plus FG-4497 plus PTK787; G+P, G-CSF plus PTK787.