Differences in BCR–TLR–NF-κB or BRAF-MAPK pathway–associated gene methylation and expression for HCL and post-GC B cells. Integrated methylation (heat map on the left) and gene expression (heat map on the right) profiles of HCL and post-GC B cells revealed enrichment of transcripts annotated for BCR–TLR–NF-κB (red) or BRAF-MAPK (blue) signaling pathways and cell adhesion (green) among the hypomethylated and upregulated genes, whereas cell-differentiation markers (purple) and genes methylated in cancer (orange) were enriched among the hypermethylated and underexpressed transcripts. All selected genes showed statistically significant changes. fc_met (purple bars) represents fold change in methylation between HCL and post-GC B cells, and fc_gep (red bars) represents fold change in gene expression between HCL and post-GC B cells. Methylation profiling (histotype_Meth) included low- and intermediate-maturity memory B cells (loMat_postGC_Bcell and inMat_postGC_Bcell), splenic MGZ B cells (MGZ_Bcell), and HCL. GEP (histotype_GEP) included memory B cells (postGC_Bcell) and HCL.