TET2 gene mutation classification, type, and clinical characteristics. (A) Scatterplot of the VAFs of patients with TET2^MT. The VAF of first-hit TET2^MT was plotted on the x-axis and that of the second-hit TET2^MT, if present, on the y-axis. Patients were categorized into 5 groups as explained in the text. The red oval corresponds to biTET2^MT cases, the gray bar to undetermined cases, the blue oval to biclonal TET2^MT cases, the light green oval to monoTET2^MT cases, and the yellow oval to hemihomozygous TET2^MT (UPD) cases. (B) Percentages of different types of TET2^MT in biTET2ⁱ cases and significance of truncating mutations vs monoTET2^MT. Fisher’s exact test was used for analysis. (C) Bar graphs showing the distribution of biTET2ⁱ and monoTET2^MT cases per diagnosis and cytogenetics. The bar columns indicate percentages. (D) Pie chart showing the percentage of cases per configuration. (E) Pie charts of biTET2ⁱ, monoTET2^MT, and TET2^WT respectively representing the percentage of cases per classification. CMML (+) indicates cases with CMML at the time of presentation, CMML (−) indicates no CMML diagnosis, monocytosis (+) indicates the presence of monocytosis, and monocytosis (−) indicates the absence of monocytosis.