Interactions of neutrophil-derived OSM with gp130-containing receptors enhance P-selectin–dependent rolling on stimulated mouse and human endothelial cells in vitro. (A) Number of mouse neutrophils rolling on MLECs 30 minutes after A23187 stimulation in the presence of the indicated mAb. (B) Mean rolling velocity of mouse neutrophils on MLECs 30 minutes after A23187 stimulation in the presence of the indicated antibody. (C) Number of human neutrophils rolling on HUVECs at the indicated time before or after adding histamine (time = 0), in the presence of the indicated antibody. (D) Mean rolling velocity of neutrophils on HUVECs 5 minutes after adding histamine, in the presence of the indicated antibody. (E) Number of neutrophils rolling on HUVECs at the indicated time before or after adding thrombin (time = 0), in the presence of the indicated antibody. (F) Mean rolling velocity of neutrophils on HUVECs 5 minutes after adding thrombin, in the presence of the indicated antibody. (G) Number of neutrophils rolling on immobilized purified P-selectin at the indicated time after initiation of perfusion, in the presence or absence of histamine, thrombin, and the indicated antibody. The data represent the mean ± SD from 3 experiments. *P < .05; **P < .01.