Differential protein expression in the BM of patients with AML compared with healthy control subjects. (A) A heatmap representation of a total of 168 differentially expressed analytes measured by SOMAscan assay using the BM plasma samples obtained from patients with AML (n = 10) and healthy control subjects (n = 10). Heatmap rows and columns were hierarchically clustered (unsupervised) within the groups. Color scale indicates relative expression, with more abundant proteins shown in red and less abundant proteins shown in blue. (B) A Venn diagram of unique and shared proteins identified as dysregulated by SOMAscan analysis of the BM plasma and PB serum samples obtained from patients with AML and healthy control subjects. Comparison of SOMAscan proteomic signatures of patients with AML vs healthy control subjects revealed 65 analytes similarly regulated and 1 analyte differently regulated in BM plasma and PB serum samples. (C) A Venn diagram illustrating the unique and overlapping differential expression events between the proteomics data obtained from SOMAscan analysis of BM plasma samples and the transcriptome data generated by in-depth bulk RNA-sequencing of whole BM aspirate cells.