High-throughput SOMAscan proteomics platform identifies differentially expressed proteins in the BM microenvironment of patients with AML. (A) A principal component analysis showing variance between the SomaLogic calibrators, SomaLogic QC samples, internal QC samples, and biological samples. (B) A volcano plot showing differentially abundant proteins in BM plasma samples obtained from patients with AML (n = 10) compared with healthy control subjects (n = 10). The SOMAscan analysis revealed a total of 168 proteins that were differentially abundant in the AML BM microenvironment compared with healthy control subjects (unpaired Mann-Whitney U test, FC ≥1.5; FDR ≤0.05). Data for proteins that were not classified as differentially expressed after filtering are plotted in gray. Data for proteins that are upregulated and downregulated are denoted by red and blue symbols, respectively.