NPM1^wtrelapse is associated with persistent mutations. (A) For 11/14 patients with NPM1^wt relapse, paired samples at diagnosis and relapse were available for sequencing. Shown are genes mutated at least once in the analyzed samples. Genes with >1 mutation/patient are shown once. (B) Number of persistent mutations at relapse; median, 2 (range, 0-4). (C) Left: mutations in 7 genes detected at diagnosis were found to persist at relapse. Fraction of persistent mutations in relation to all 11 patients is shown. Right: The 4 most frequently mutated genes at D and R are shown as heat maps to visualize persistence of mutations. DNMT3A is the most prevalent mutation and also the most stable mutation over time. (D) The variant allele frequency of the indicated genes is given at D and R. D, diagnosis; R, relapse.