Novel functional markers for CD16⁺and CD16⁻MDDCs. MDDC subsets were generated and exposed to media (immature), LPS (mature), or HIV for 24 hours, as described in Figure 1A. Shown are real-time RT-PCR validation of relative ITGAE/CD103, CDH1/E-cadherin, ALDH1A2/RALDH2, and TCF7L2/TCF4 mRNA expression in immature MDDC subsets (A), as well as IGSF2/CD101 and SIGLEC1/CD169 in HIV-exposed MDDC subsets (B). (A-B) Results were generated with matched MDDC subsets from 3 to 5 different donors. Each symbol represents the median value of 2 to 3 RT-PCR replicates, with values for CD16⁻ MDDCs being considered 1. Paired Student t test P values are indicated on the graphs. (C) Shown are levels of TNF-α, CCL22, and CCL18 quantified by ELISA in cell culture supernatants from matched CD16⁺ and CD16⁻ MDDCs on exposure to media, LPS, or HIV (n = 4-5, mean ± SEM). Paired Student t test P values for log₁₀ cytokine levels are indicated on the graphs.