Differential gene expression in CD16⁺and CD16⁻MDDCs in response to LPS. Transcriptional profiling was performed as described in Figure 1A,E-F with matched MDDC subsets exposed to media (immature) or LPS (mature) for 24 hours. Shown are top-regulated genes in immature and mature CD16⁺ compared with CD16⁻ MDDCs (A) and differentially expressed genes in CD16⁺ vs CD16⁻ MDDC subsets exposed to LPS (P < .05; FC cutoff, 1.3) linked to the gene ontology terms chemotaxis (B) and cytokines (C). Heat map cells are scaled by the expression level z-scores for each probe individually. Results were generated with cells from 5 different donors identified with different color codes.