Gene ontology classification of differentially expressed genes in immature CD16⁺and CD16⁻MDDCs. Transcriptional profiling was performed as described in Figure 1A,D. Differentially expressed genes in CD16⁺ (green) vs CD16⁻ (blue) MDDCs exposed to media (immature; P < .05; FC cutoff, 1.3) were classified using gene ontology in (A) transcription factors, (B) cytokines, (C) adhesion molecules, (D) chemotaxis, and (E) cell projections. Each heat map column represents data from a distinct donor for matched immature CD16⁺ vs CD16⁻ MDDCs (n = 5).