Findings from next-generation gene expression studies in MYD88^WTWM. (A) The top 100 most statistically significant genes between samples from 18 MYD88^WT and 75 MYD88^MUT patients are shown, demonstrating a uniform gene signature associated with the MYD88^WT population. (B) Principal component analysis of the top 500 high variance genes revealed a clustering of MYD88^WT and MYD88^MUT WM samples, regardless of CXCR4 mutation status that was distinct from healthy donor peripheral blood B, memory B, and plasma cells. (C) These findings were also recapitulated in the supervised clustering of the top 100 most statistically significant differentially expressed genes between healthy donor memory B cells and MYD88^WT WM samples, in which gene expression levels were very similar between all WM samples regardless of MYD88 and CXCR4 mutation status.