Proposed model for enhanced Mk maturation based on HEL cell data. MRTFA^OE promotes more robust Mk maturation by enhancing cytoskeleton-associated gene expression. Left: Normal Mk maturation with TPA promotes the binding of TCF factors (eg, ETS factors) to SRF dimers poised on chromatin and the binding of SRF to upregulate genes. Right: With MRTFA^OE- and TPA-induced Mk maturation, more MRTFA is bound to poised dimers of SRF that both promotes SRF binding and upregulates cytoskeletal genes. At the same time, it also reduces regulation by the SRF/TCF axis. Dotted arrow on the lower right indicates variable effects on mRNA levels. CArG, CCWWWWWWGG; ETS, ETS binding site, GGAA.