Figure 4.
Figure 4. Macrophage infusion improved medullar erythropoiesis. (A) Representative plots of flow cytometric analysis of the erythroblast populations in the BM of WT and Hmox1 KO mice without or with macrophage infusion using FITC-CD71 and APC-Ter119 antibodies. The animals were transcardially perfused with PBS before harvesting the BM cells and analysis of the cell populations. (B) The percentages of CD71high/Ter119high erythroblast cells from flow cytometric analysis. All samples were collected at 12 weeks after macrophage infusion. Statistical analyses were performed using 2-way ANOVA (multiple comparisons). The graph was prepared with GraphPad Prism software. **P < .01, ***P < .001. APC-H, allophycocyanin-height; FITC-H, fluorescein isothiocyanate–height.

Macrophage infusion improved medullar erythropoiesis. (A) Representative plots of flow cytometric analysis of the erythroblast populations in the BM of WT and Hmox1 KO mice without or with macrophage infusion using FITC-CD71 and APC-Ter119 antibodies. The animals were transcardially perfused with PBS before harvesting the BM cells and analysis of the cell populations. (B) The percentages of CD71high/Ter119high erythroblast cells from flow cytometric analysis. All samples were collected at 12 weeks after macrophage infusion. Statistical analyses were performed using 2-way ANOVA (multiple comparisons). The graph was prepared with GraphPad Prism software. **P < .01, ***P < .001. APC-H, allophycocyanin-height; FITC-H, fluorescein isothiocyanate–height.

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