Macrophage infusion prevented pathological accumulation of iron in kidneys. (A) Kidneys (bottom panel) and Perls’ Prussian Blue iron staining of kidney sections (top panel) of WT, Hmox1 KO mice without or with macrophage infusion. Scale bar, 100 μm. (B) Nonheme iron in kidneys of WT and Hmox1 KO mice without or with macrophage infusion. (C) Western blot analysis of ferritin levels in kidneys of WT and Hmox1 KO mice without or with macrophage (Mac) infusion. qRT-PCR analyses of (D) TfR1 (n = 4), (E) erythropoietin (Epo) (n = 5), and (F) CD163 mRNA (n = 4) levels in the kidneys of WT and Hmox1 KO mice without or with macrophage infusion. All animals were infused with macrophages or PBS for 12 weeks. Mean ± SD; statistical analyses were performed using 2-way ANOVA (multiple comparisons). The graphs were created with GraphPad Prism software. *P < .05, **P < .01, ***P < .001.